Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Effect of 10 Gy X‐ray on viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells. Blue: untreated controls; Pink: irradiated cells. Cell counts were conducted at 24, 48, and 72 h post‐treatment. Statistical analysis was performed using two‐way ANOVA with Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Note: Y ‐axes are scaled independently for each cell line to account for differences in baseline growth, allowing clearer visualization of treatment effects. Tumor cell counts and representative images can be found in Appendix Table and Figure .

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques: Irradiation

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of untreated NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells and cells exposed to X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells after treatment with IMP‐1700 (5 µM), ciprofloxacin (CPX) (15 µM), their combination, or DMSO, with and without 10 Gy X‐ray. Cell counts were measured at 24, 48, and 72 h post‐treatment. Statistical significance assessed via two‐way ANOVA and Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Significant differences are only shown compared to untreated or 10 Gy. Tumor cell count values and images are available in Appendix Table and Figure , , , .

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques: Cell Characterization

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700, with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700 and ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with DMSO, with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: Gels

Article Title: Tendon dECM Composited with Chitosan with Loading Skin Precursor Stem Cell Exosome for Enhanced Diabetic Wound Healing

doi: 10.3390/gels12050361

Figure Lengend Snippet: The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

Article Snippet: The cytocompatibility of dECM@QCS and exo@dECM@QCS composite hydrogels was investigated by culturing with mouse embryonic fibroblast cells (NIH 3T3, ATCC, CRL-1658TM, Manassas, VA, USA) using fluorescent images and Cell Counting Kit-8 (CCK-8) assay [ ].

Techniques: Activity Assay, Staining, Wound Healing Assay, Migration, CCK-8 Assay